TY - JOUR
T1 - Susceptibility to acyclovir of herpes simplex virus isolates obtained between 1977 and 1996 in Japan
AU - Hasegawa, Tomomi
AU - Kawana, Takashi
AU - Okuda, Tomoko
AU - Horii, Mitsue
AU - Tsukada, Tokie
AU - Shiraki, Kimiyasu
PY - 2001
Y1 - 2001
N2 - The susceptibility of genital herpes to acyclovir (ACV) in immunocompetent women was examined, as was the frequency of ACV-resistant viruses by analyzing 56 clinical isolates in Japan between 1977 and 1996. The mean susceptibilities of herpes simplex virus (HSV) type 1 and type 2 were 0.13±0.74 and 0.42±0.14 μg/ml, respectively, assessed by the 50% inhibitory concentration of plaque formation. The susceptibility to ACV of clinical isolates did not changed since 1977, and also that of nine pairs of HSV-1 and HSV-2 isolates was not affected by ACV treatment. In order to characterize the change in the virus population, the quantitation of the ACV-resistant virus in 104 plaque forming units (PFU) of clinical isolates was adopted. The mean frequencies of ACV-resistant viruses per 104 PFU for all strains of HSV-1 and HSV-2 were 0.31±0.41 and 9.74±14.83, respectively, and were not influenced by ACV treatment. Additionally, the phenotypes of ACV-resistance were not influenced by ACV treatment, and more than 90% of ACV-resistant viruses were found to be thymidine kinase-deficient. This study characterized clinical isolates with respect to ACV susceptibility as a population and the quantitative and qualitative characterization of ACV-resistant virus in the virus population of clinical isolates was also studied. The susceptibility of isolates from genital lesions, the frequency of ACV-resistant viruses, and also the phenotypic characterization of ACV-resistant viruses was maintained between 1977 and 1996, even after the introduction of ACV treatment for genital herpes in Japan. (C) 2001 Wiley-Liss, Inc.
AB - The susceptibility of genital herpes to acyclovir (ACV) in immunocompetent women was examined, as was the frequency of ACV-resistant viruses by analyzing 56 clinical isolates in Japan between 1977 and 1996. The mean susceptibilities of herpes simplex virus (HSV) type 1 and type 2 were 0.13±0.74 and 0.42±0.14 μg/ml, respectively, assessed by the 50% inhibitory concentration of plaque formation. The susceptibility to ACV of clinical isolates did not changed since 1977, and also that of nine pairs of HSV-1 and HSV-2 isolates was not affected by ACV treatment. In order to characterize the change in the virus population, the quantitation of the ACV-resistant virus in 104 plaque forming units (PFU) of clinical isolates was adopted. The mean frequencies of ACV-resistant viruses per 104 PFU for all strains of HSV-1 and HSV-2 were 0.31±0.41 and 9.74±14.83, respectively, and were not influenced by ACV treatment. Additionally, the phenotypes of ACV-resistance were not influenced by ACV treatment, and more than 90% of ACV-resistant viruses were found to be thymidine kinase-deficient. This study characterized clinical isolates with respect to ACV susceptibility as a population and the quantitative and qualitative characterization of ACV-resistant virus in the virus population of clinical isolates was also studied. The susceptibility of isolates from genital lesions, the frequency of ACV-resistant viruses, and also the phenotypic characterization of ACV-resistant viruses was maintained between 1977 and 1996, even after the introduction of ACV treatment for genital herpes in Japan. (C) 2001 Wiley-Liss, Inc.
KW - ACV
KW - ACV resistance
KW - Genital herpes
KW - HSV
KW - Susceptibility
UR - http://www.scopus.com/inward/record.url?scp=0035218351&partnerID=8YFLogxK
U2 - 10.1002/1096-9071(200101)63:1<57::AID-JMV1008>3.0.CO;2-9
DO - 10.1002/1096-9071(200101)63:1<57::AID-JMV1008>3.0.CO;2-9
M3 - 学術論文
C2 - 11130888
AN - SCOPUS:0035218351
SN - 0146-6615
VL - 63
SP - 57
EP - 63
JO - Journal of Medical Virology
JF - Journal of Medical Virology
IS - 1
ER -