抄録
D-amino acids have distinct roles from their L-enantiomer. In particular, some D-amino acids function as agonists or antagonists of neuronal receptors and are involved in higher brain functions. Thus, it is important to precisely measure the levels of these amino acid enantiomers in cells and tissues. Various quantification methods have been developed for measurements of chiral amino acids. However, each method has advantages and disadvantages. Additionally, measuring the amino acid enantiomers in crude biological samples requires a higher selectivity. In this study, we developed a quantification method for amino acid enantiomers using derivatization with Nα-(5-Fluoro-2,4-dinitrophenyl)-L-leucinamide (L-FDLA) followed by liquid chromatography–tandem mass spectrometry (LC/MS/MS) with a conventional reversed-phase column. We simultaneously identified 10 chiral amino acids. Furthermore, we applied this method to investigate murine tissue samples and examined the effect of aging on the amino acid levels in aged brain regions. We found that aging decreased the levels of both D-serine and D-aspartate in the hippocampus. In addition, D-Phenylalanine in the thalamus significantly increased with age. In conclusion, our method is suitable for the quantification of the D-amino acids in crude biological samples and may contribute to elucidating the biological roles of chiral amino acids.
本文言語 | 英語 |
---|---|
論文番号 | 57 |
ページ(範囲) | 1-13 |
ページ数 | 13 |
ジャーナル | Metabolites |
巻 | 11 |
号 | 1 |
DOI | |
出版ステータス | 出版済み - 2021/01 |
ASJC Scopus 主題領域
- 内分泌学、糖尿病および代謝内科学
- 生化学
- 分子生物学