Fluorescent Labeling of a Target Protein with an Alkyl Diazirine Photocrosslinker Bearing a Cinnamate Moiety

Taikai Nakashima, Takumi Iwanabe, Hiroki Tanimoto, Takenori Tomohiro*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

1 被引用数 (Scopus)

抄録

A novel fluorogenic alkyl diazirine photocrosslinker bearing an o-hydroxycinnamate moiety has been developed for identification of the targets of bioactive molecules. The o-hydroxycinnamate moiety can be converted to the corresponding 7-hydroxycoumarin derivative, which should be created on the interacting site within the photocaptured target protein. The label yield and fluorescence intensity have been immensely improved in comparison with our previous aromatic crosslinkers to facilitate target identification in small quantities.

本文言語英語
論文番号e202400288
ジャーナルChemistry - An Asian Journal
19
11
DOI
出版ステータス出版済み - 2024/06/03

ASJC Scopus 主題領域

  • 生化学
  • 化学一般
  • 有機化学

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