@inbook{fd2ec7ac636e4145810fd36ea85bf41d,
title = "Engineering of plant type III polyketide synthases",
abstract = "Members of the chalcone synthase superfamily of type III polyketide synthases (PKSs) catalyze iterative condensations of CoA thioesters to produce a variety of polyketide scaffolds with remarkable structural diversity and biological activities. The homodimeric type III PKSs share a common three-dimensional overall fold with a conserved Cys-His-Asn catalytic triad; notably, only a slight modification of the active site dramatically expands the catalytic repertoire of the enzymes. In addition, the enzymes exhibit extremely promiscuous substrate specificities, and accept a variety of nonphysiological substrates, making the type III PKSs an excellent platform for the further production of unnatural, novel polyketide scaffolds with promising biological activities. This chapter summarizes recent advances in the engineering of plant type III PKS enzymes in our laboratories, using approaches combining structure-based enzyme engineering and precursor-directed biosynthesis with rationally designed substrate analogs.",
keywords = "Chalcone synthase superfamily enzyme, Enzyme engineering, Polyketide synthase, Precursor-directed biosynthesis, Structure-based engineering, Unnatural natural product",
author = "Toshiyuki Wakimoto and Hiroyuki Morita and Ikuro Abe",
note = "Funding Information: The authors would like to express their sincere appreciation to the excellent coworkers whose contributions are cited in the text. This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and by the PRESTO and CREST programs from Japan Science and Technology Agency, Japan.",
year = "2012",
doi = "10.1016/B978-0-12-394290-6.00016-1",
language = "英語",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "337--358",
booktitle = "Methods in Enzymology",
}