Effects of microbubbles on ultrasound-mediated gene transfer in human prostate cancer PC3 cells: Comparison among Levovist, YM454, and MRX-815H

Akihiko Watanabe; Remon Otake; Tetsuo Nozaki; Akihiro Morii; Ryohei Ogawa; Shinichi Fujimoto; Shinobu Nakamura; Hideki Fuse; Takashi Kondo

doi:10.1016/j.canlet.2008.02.004

Effects of microbubbles on ultrasound-mediated gene transfer in human prostate cancer PC3 cells: Comparison among Levovist, YM454, and MRX-815H

Akihiko Watanabe, Remon Otake, Tetsuo Nozaki, Akihiro Morii, Ryohei Ogawa^*, Shinichi Fujimoto, Shinobu Nakamura, Hideki Fuse, Takashi Kondo

^*この論文の責任著者

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

19 被引用数 (Scopus)

抄録

We compared the enhancement effects of three different echo contrast agents (ECAs); Levovist, YM454, and MRX-815H as artificial microbubbles on ultrasound mediated gene transfection (USMGT) with 1 MHz ultrasound at 0.2 MPa using a luciferase expression vector in PC3 cells and elucidated the mechanisms of differences of USMGT facilitation by these ECAs. At a concentration of each ECA that induced iso-survival, ECAs with lipid shell (YM454 and MRX-815H) facilitated USMGT higher than those without shell (Levovist), and the order of the ECAs facilitating free radical formation by sonication was; YM454 > MRX-815H > Levovist. These results suggested that the lipid shell type ECAs facilitated gene transfer higher than that by the non-shell type ECA.

本文言語	英語
ページ（範囲）	107-112
ページ数	6
ジャーナル	Cancer Letters
巻	265
号	1
DOI	https://doi.org/10.1016/j.canlet.2008.02.004
出版ステータス	出版済み - 2008/06/28

ASJC Scopus 主題領域

腫瘍学
癌研究

UN SDG

この成果は、次の持続可能な開発目標に貢献しています

文献へのアクセス

10.1016/j.canlet.2008.02.004

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引用スタイル

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title = "Effects of microbubbles on ultrasound-mediated gene transfer in human prostate cancer PC3 cells: Comparison among Levovist, YM454, and MRX-815H",

abstract = "We compared the enhancement effects of three different echo contrast agents (ECAs); Levovist, YM454, and MRX-815H as artificial microbubbles on ultrasound mediated gene transfection (USMGT) with 1 MHz ultrasound at 0.2 MPa using a luciferase expression vector in PC3 cells and elucidated the mechanisms of differences of USMGT facilitation by these ECAs. At a concentration of each ECA that induced iso-survival, ECAs with lipid shell (YM454 and MRX-815H) facilitated USMGT higher than those without shell (Levovist), and the order of the ECAs facilitating free radical formation by sonication was; YM454 > MRX-815H > Levovist. These results suggested that the lipid shell type ECAs facilitated gene transfer higher than that by the non-shell type ECA.",

keywords = "Echo contrast agent, Microbubble, Sonication, Transfection, Ultrasonic",

author = "Akihiko Watanabe and Remon Otake and Tetsuo Nozaki and Akihiro Morii and Ryohei Ogawa and Shinichi Fujimoto and Shinobu Nakamura and Hideki Fuse and Takashi Kondo",

year = "2008",

month = jun,

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doi = "10.1016/j.canlet.2008.02.004",

language = "英語",

volume = "265",

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TY - JOUR

T1 - Effects of microbubbles on ultrasound-mediated gene transfer in human prostate cancer PC3 cells

T2 - Comparison among Levovist, YM454, and MRX-815H

AU - Watanabe, Akihiko

AU - Otake, Remon

AU - Nozaki, Tetsuo

AU - Morii, Akihiro

AU - Ogawa, Ryohei

AU - Fujimoto, Shinichi

AU - Nakamura, Shinobu

AU - Fuse, Hideki

AU - Kondo, Takashi

PY - 2008/6/28

Y1 - 2008/6/28

N2 - We compared the enhancement effects of three different echo contrast agents (ECAs); Levovist, YM454, and MRX-815H as artificial microbubbles on ultrasound mediated gene transfection (USMGT) with 1 MHz ultrasound at 0.2 MPa using a luciferase expression vector in PC3 cells and elucidated the mechanisms of differences of USMGT facilitation by these ECAs. At a concentration of each ECA that induced iso-survival, ECAs with lipid shell (YM454 and MRX-815H) facilitated USMGT higher than those without shell (Levovist), and the order of the ECAs facilitating free radical formation by sonication was; YM454 > MRX-815H > Levovist. These results suggested that the lipid shell type ECAs facilitated gene transfer higher than that by the non-shell type ECA.

AB - We compared the enhancement effects of three different echo contrast agents (ECAs); Levovist, YM454, and MRX-815H as artificial microbubbles on ultrasound mediated gene transfection (USMGT) with 1 MHz ultrasound at 0.2 MPa using a luciferase expression vector in PC3 cells and elucidated the mechanisms of differences of USMGT facilitation by these ECAs. At a concentration of each ECA that induced iso-survival, ECAs with lipid shell (YM454 and MRX-815H) facilitated USMGT higher than those without shell (Levovist), and the order of the ECAs facilitating free radical formation by sonication was; YM454 > MRX-815H > Levovist. These results suggested that the lipid shell type ECAs facilitated gene transfer higher than that by the non-shell type ECA.

KW - Echo contrast agent

KW - Microbubble

KW - Sonication

KW - Transfection

KW - Ultrasonic

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DO - 10.1016/j.canlet.2008.02.004

M3 - 学術論文

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AN - SCOPUS:43449136747

SN - 0304-3835

VL - 265

SP - 107

EP - 112

JO - Cancer Letters

JF - Cancer Letters

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