Cellular localization of a putative Na+/H+ exchanger 3 during ontogeny in the pronephros and mesonephros of the Japanese black salamander (Hynobius nigrescens Stejneger)

Tomoko Kumano, Norifumi Konno, Tatsuya Wakasugi, Kouhei Matsuda, Hideki Yoshizawa, Minoru Uchiyama*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

10 被引用数 (Scopus)

抄録

The cloning of cDNA and an examination of the tissue distribution of Na+/H+ exchanger 3 (NHE3) were carried out in the Japanese black salamander, Hynobius nigrescens. The cellular localization of Hynobius NHE3 was examined by in situ hybridization and immunohistochemistry during ontogeny in the nephron of the pronephros and mesonephros of the salamander. The partial amino acid sequence of Hynobius NHE3 was 81% and 72% identical to rat NHE3 and stingray NHE3, respectively. Hynobius NHE3 mRNA and protein were exclusively expressed along the late portion of the distal tubule to the anterior part of the pronephric duct of premetamorphic larvae (IY stages 43-50). NHE3 mRNA was expressed in the pronephros but not in the external gills in the larvae at the digit differentiation stage (IY stage 50). In the adult, mRNA was strongly expressed in the mesonephros but not in the ventral and dorsal skin. In juvenile and adult specimens, NHE3 immunoreactivity was observed at the apical membrane of the initial parts of the distal tubules of the mesonephric kidney. Immunohistochemical and in situ hybridization studies suggested that Na + absorption coupled with H+ secretion via NHE3 occurred in the distal nephron of the pronephros and mesonephros. This is the first study to indicate NHE3 expression during ontogeny in amphibians.

本文言語英語
ページ(範囲)675-685
ページ数11
ジャーナルCell and Tissue Research
331
3
DOI
出版ステータス出版済み - 2008/03

ASJC Scopus 主題領域

  • 病理学および法医学
  • 組織学
  • 細胞生物学

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