TY - JOUR
T1 - Uncovering the metabolomic effects of Kampo formulas using 1H-NMR spectroscopy
T2 - A case study of goreisan
AU - Awale, Suresh
AU - Jo, Michiko
AU - Watanabe, Shiro
AU - Shibahara, Naotoshi
AU - Matsumoto, Kinzo
N1 - Publisher Copyright:
© 2023 Japan Society for Oriental Medicine and Japan Society of Medical and Pharmaceutical Sciences for Traditional Medicine.
PY - 2023/8
Y1 - 2023/8
N2 - Aim: The purpose of this study was to explore the effects of goreisan (GRS) on rats through the analysis of 1H-NMR of urine samples, to assess the variations in metabolites affected by GRS, and to gain a new metabolomic understanding of the effects of Kampo medicine. Methods: Male Wistar rats were randomly divided into two groups: a control group that was fed a standard diet, and a GRS group that was fed a 1% GRS diet. The study was conducted over a four-week period. At the end of the experiment, urine samples were analyzed using 1H-NMR spectroscopy to evaluate the metabolic changes induced by GRS administration. Additionally, various blood parameters were also analyzed. Results: Administration of GRS resulted in an elevation of lactate, acetate, and other metabolites, such as trimethylamine-N-oxide and hippurate, in urine. Additionally, there was a significant decrease in the blood urea nitrogen (BUN) level in the blood of the GRS-treated group. Conclusion: This study has highlighted the substantial changes in lactate, acetate, and other urine metabolites caused by GRS. The findings demonstrate the effectiveness of 1H-NMR spectroscopy in assessing metabolic alterations in response to the therapeutic application of a Kampo formula. This research contributes to the knowledge of mechanisms of action from a metabolomic point of view and offers fresh insights into the effects of complex Kampo formulas such as GRS, using 1H-NMR spectroscopic techniques.
AB - Aim: The purpose of this study was to explore the effects of goreisan (GRS) on rats through the analysis of 1H-NMR of urine samples, to assess the variations in metabolites affected by GRS, and to gain a new metabolomic understanding of the effects of Kampo medicine. Methods: Male Wistar rats were randomly divided into two groups: a control group that was fed a standard diet, and a GRS group that was fed a 1% GRS diet. The study was conducted over a four-week period. At the end of the experiment, urine samples were analyzed using 1H-NMR spectroscopy to evaluate the metabolic changes induced by GRS administration. Additionally, various blood parameters were also analyzed. Results: Administration of GRS resulted in an elevation of lactate, acetate, and other metabolites, such as trimethylamine-N-oxide and hippurate, in urine. Additionally, there was a significant decrease in the blood urea nitrogen (BUN) level in the blood of the GRS-treated group. Conclusion: This study has highlighted the substantial changes in lactate, acetate, and other urine metabolites caused by GRS. The findings demonstrate the effectiveness of 1H-NMR spectroscopy in assessing metabolic alterations in response to the therapeutic application of a Kampo formula. This research contributes to the knowledge of mechanisms of action from a metabolomic point of view and offers fresh insights into the effects of complex Kampo formulas such as GRS, using 1H-NMR spectroscopic techniques.
KW - Goreisan
KW - NMR
KW - metabolomics
KW - urine
UR - http://www.scopus.com/inward/record.url?scp=85170630535&partnerID=8YFLogxK
U2 - 10.1002/tkm2.1369
DO - 10.1002/tkm2.1369
M3 - 学術論文
AN - SCOPUS:85170630535
SN - 2053-4515
VL - 10
SP - 142
EP - 149
JO - Traditional & Kampo Medicine
JF - Traditional & Kampo Medicine
IS - 2
ER -