Synthesis of novel caged antisense oligonucleotides with fluorescence property

Shin Hikage, Yasuo Sasaki, Terunobu Hisai, Hiroki Tanimoto, Tsumoru Morimoto, Yasuhiro Nishiyama*, Kiyomi Kakiuchi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

In this study, novel caged antisense oligonucleotides with thiochromone S,S-dioxide as the photolabile protecting group were synthesized, and their photodeprotection was investigated. In vitro experiment, the original target molecule was successfully recovered by photoirradiation. Furthermore, the photodeprotection process was also successfully monitored by the fluorescence spectroscopy of highly fluorescent byproducts generated from the thiochromone-type photolabile protecting group.

Original languageEnglish
Pages (from-to)175-183
Number of pages9
JournalJournal of Photochemistry and Photobiology A: Chemistry
Volume331
DOIs
StatePublished - 2016/12/01

Keywords

  • Antisense oligonucleotide
  • Caged compound
  • Fluorescence
  • Luciferin–luciferase reaction
  • Thiochromone

ASJC Scopus subject areas

  • General Chemistry
  • General Chemical Engineering
  • General Physics and Astronomy

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