Real-time visualization of intratumoral necrosis using split-luciferase reconstitution by protein trans-splicing

Go Kagiya; Ayaka Sato; Ryohei Ogawa; Masanori Hatashita; Mana Kato; Makoto Kubo; Fumiaki Kojima; Fumitaka Kawakami; Yukari Nishimura; Naoya Abe; Fuminori Hyodo

doi:10.1016/j.omto.2020.12.001

Real-time visualization of intratumoral necrosis using split-luciferase reconstitution by protein trans-splicing

Go Kagiya, Ayaka Sato, Ryohei Ogawa^*, Masanori Hatashita, Mana Kato, Makoto Kubo, Fumiaki Kojima, Fumitaka Kawakami, Yukari Nishimura, Naoya Abe, Fuminori Hyodo

^*Corresponding author for this work

Diagnostic and Therapeutic Radiology

Research output: Contribution to journal › Article › peer-review

Abstract

Necrosis occurs with the development of various diseases and in tumor tissue responding to cancer treatment. To evaluate the preclinical effectiveness of different anticancer drugs and radiation therapy, Kagiya and colleagues have constructed a real-time visualization system for intratumoral necrosis using split-luciferase reconstitution by protein trans-splicing.

Original language	English
Pages (from-to)	48-58
Number of pages	11
Journal	Molecular Therapy - Oncolytics
Volume	20
DOIs	https://doi.org/10.1016/j.omto.2020.12.001
State	Published - 2021/03/26

Keywords

bioluminescent imaging
cell death
extein
intein
necrosis
necrosis imaging reporter
protein trans-splicing
split-luciferase reconstitution

ASJC Scopus subject areas

Molecular Medicine
Oncology
Cancer Research
Pharmacology (medical)

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10.1016/j.omto.2020.12.001

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title = "Real-time visualization of intratumoral necrosis using split-luciferase reconstitution by protein trans-splicing",

abstract = "Necrosis occurs with the development of various diseases and in tumor tissue responding to cancer treatment. To evaluate the preclinical effectiveness of different anticancer drugs and radiation therapy, Kagiya and colleagues have constructed a real-time visualization system for intratumoral necrosis using split-luciferase reconstitution by protein trans-splicing.",

keywords = "bioluminescent imaging, cell death, extein, intein, necrosis, necrosis imaging reporter, protein trans-splicing, split-luciferase reconstitution",

author = "Go Kagiya and Ayaka Sato and Ryohei Ogawa and Masanori Hatashita and Mana Kato and Makoto Kubo and Fumiaki Kojima and Fumitaka Kawakami and Yukari Nishimura and Naoya Abe and Fuminori Hyodo",

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year = "2021",

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doi = "10.1016/j.omto.2020.12.001",

language = "英語",

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journal = "Molecular Therapy - Oncolytics",

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TY - JOUR

T1 - Real-time visualization of intratumoral necrosis using split-luciferase reconstitution by protein trans-splicing

AU - Kagiya, Go

AU - Sato, Ayaka

AU - Ogawa, Ryohei

AU - Hatashita, Masanori

AU - Kato, Mana

AU - Kubo, Makoto

AU - Kojima, Fumiaki

AU - Kawakami, Fumitaka

AU - Nishimura, Yukari

AU - Abe, Naoya

AU - Hyodo, Fuminori

PY - 2021/3/26

Y1 - 2021/3/26

N2 - Necrosis occurs with the development of various diseases and in tumor tissue responding to cancer treatment. To evaluate the preclinical effectiveness of different anticancer drugs and radiation therapy, Kagiya and colleagues have constructed a real-time visualization system for intratumoral necrosis using split-luciferase reconstitution by protein trans-splicing.

AB - Necrosis occurs with the development of various diseases and in tumor tissue responding to cancer treatment. To evaluate the preclinical effectiveness of different anticancer drugs and radiation therapy, Kagiya and colleagues have constructed a real-time visualization system for intratumoral necrosis using split-luciferase reconstitution by protein trans-splicing.

KW - bioluminescent imaging

KW - cell death

KW - extein

KW - intein

KW - necrosis

KW - necrosis imaging reporter

KW - protein trans-splicing

KW - split-luciferase reconstitution

UR - http://www.scopus.com/inward/record.url?scp=85100045076&partnerID=8YFLogxK

U2 - 10.1016/j.omto.2020.12.001

DO - 10.1016/j.omto.2020.12.001

M3 - 学術論文

C2 - 33575470

AN - SCOPUS:85100045076

SN - 2372-7705

VL - 20

SP - 48

EP - 58

JO - Molecular Therapy - Oncolytics

JF - Molecular Therapy - Oncolytics

ER -

Real-time visualization of intratumoral necrosis using split-luciferase reconstitution by protein trans-splicing

Abstract

Keywords

ASJC Scopus subject areas

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