Quantitative Photodeprotection Assessment of Caged Resveratrol by Fluorescence Measurement

Shin Hikage, Yasuhiro Nishiyama*, Yasuo Sasaki, Hiroki Tanimoto, Tsumoru Morimoto, Kiyomi Kakiuchi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

For monitoring the regenerated bioactivity of a masked bioactive compound, resveratrol (a luciferase inhibitor) was selected to target such a compound. Caged resveratrol, masked by thiochromone-type photolabile-protecting groups was synthesized in the study. Each caged resveratrol showed lower bioactivity when compared to that shown by the original molecule. After photoirradiation, the original bioactivity was found to be regenerated. Furthermore, the fluorescent compound derived from the thiochromone-type photolabile-protecting groups was generated simultaneously. A linear correlation was observed between the regenerated bioactivity and generated fluorescence intensity. Thus, we quantitatively monitored the recovered bioactivity successfully by measuring the fluorescence.

Original languageEnglish
Pages (from-to)2300-2307
Number of pages8
JournalACS Omega
Volume2
Issue number5
DOIs
StatePublished - 2017/05/31

ASJC Scopus subject areas

  • General Chemistry
  • General Chemical Engineering

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