TY - JOUR
T1 - Protumor role of estrogen receptor expression in oral squamous cell carcinoma cells
AU - Akyu (Takei), Rie
AU - Tomihara, Kei
AU - Yamazaki, Manabu
AU - Moniruzzaman, Rohan
AU - Heshiki, Wataru
AU - Sekido, Katsuhisa
AU - Tachinami, Hidetake
AU - Sakurai, Kotaro
AU - Yonesi, Amirmoezz
AU - Imaue, Shuichi
AU - Fujiwara, Kumiko
AU - Noguchi, Makoto
N1 - Publisher Copyright:
© 2021 Elsevier Inc.
PY - 2021/11
Y1 - 2021/11
N2 - Objective: Accumulating evidence has demonstrated the protumor role of estrogen receptor (ER)–mediated signaling in multiple cancer types, which is distinct from this signaling in sex steroid–dependent organs. However, its role in oral squamous cell carcinoma (OSCC) remains unclear. Study Design: We assessed the expression of ERα and ERβ in human OSCC tissues by immunohistochemistry and evaluated the expression of both receptors in OSCC cell lines by immunoblotting and flow cytometry. To further assess the contribution of ER-mediated signals to oral cancer progression, proliferation, invasion, and chemosensitivity, cell lines were stimulated with the ER agonist β-estradiol. Results: Immunohistochemical analysis of OSCC tissues showed that ERβ was present in the cytoplasm and nuclei of OSCC cells. In contrast, ERα was not detected in any of the cases analyzed. Additionally, the proliferation and invasiveness of OSCC cells were significantly elevated following stimulation with β-estradiol. Chemotherapeutic agent–induced apoptosis of cancer cells was attenuated by pretreatment with β-estradiol. Conclusions: ER-mediated signaling plays a crucial role in oral cancer progression by facilitating the proliferation, invasion, and chemoresistance of OSCC cells, indicating its potential for developing novel targeted therapies for this type of cancer.
AB - Objective: Accumulating evidence has demonstrated the protumor role of estrogen receptor (ER)–mediated signaling in multiple cancer types, which is distinct from this signaling in sex steroid–dependent organs. However, its role in oral squamous cell carcinoma (OSCC) remains unclear. Study Design: We assessed the expression of ERα and ERβ in human OSCC tissues by immunohistochemistry and evaluated the expression of both receptors in OSCC cell lines by immunoblotting and flow cytometry. To further assess the contribution of ER-mediated signals to oral cancer progression, proliferation, invasion, and chemosensitivity, cell lines were stimulated with the ER agonist β-estradiol. Results: Immunohistochemical analysis of OSCC tissues showed that ERβ was present in the cytoplasm and nuclei of OSCC cells. In contrast, ERα was not detected in any of the cases analyzed. Additionally, the proliferation and invasiveness of OSCC cells were significantly elevated following stimulation with β-estradiol. Chemotherapeutic agent–induced apoptosis of cancer cells was attenuated by pretreatment with β-estradiol. Conclusions: ER-mediated signaling plays a crucial role in oral cancer progression by facilitating the proliferation, invasion, and chemoresistance of OSCC cells, indicating its potential for developing novel targeted therapies for this type of cancer.
UR - http://www.scopus.com/inward/record.url?scp=85114711500&partnerID=8YFLogxK
U2 - 10.1016/j.oooo.2021.04.006
DO - 10.1016/j.oooo.2021.04.006
M3 - 学術論文
C2 - 34518137
AN - SCOPUS:85114711500
SN - 2212-4403
VL - 132
SP - 549
EP - 565
JO - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
JF - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
IS - 5
ER -