New 19(10 → 9)abeo-euphane-type triterpenoids from Trichilia dregeana leaves and NO production inhibitory activities

Yves M. Mba Nguekeu, Takeshi Kodama, Marie G. Tsepeupon Matchide, Kiep Minh Do, Josker Nghokeng, Gaétan T. Tabakam, Kiyoshi Tsuge, Silvère A. Ngouela, Tene Mathieu, Maurice Ducret Awouafack*, Hiroyuki Morita

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Phytochemical investigation of the EtOAc soluble fraction from leaves of Trichilia dregeana Sond. (Meliaceae) afforded naturally rare four new pentacyclic triterpenoids (1–4), together with five known pentacyclic analogs (5–8, and 11) and two steroids (9 and 10). Their structures were elucidated by extensive spectroscopic techniques such as 1D and 2D NMR and HRESIMS data analyses. The absolute configuration of 1 was determined by using the single-crystal X-ray diffraction analysis. The nitric oxide (NO) production inhibitory assay indicated that the EtOAc fraction as well as 4 and 7 inhibited the NO production in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells with the IC50 values of 83.53 μg/mL and 81.31 and 85.71 μM, respectively. Compounds 1–4 are rare 19(10 → 9)abeo-euphane-type triterpenoids bearing a 3,10-ether bridge. To the best of our knowledge, this study is the first isolation of triterpenoids with the 3,10-ether bridge in their skeleton from the genus Trichilia, providing new insights into the chemodiversity of the terpenoids in T. dregeana.

Original languageEnglish
Article number106001
JournalFitoterapia
Volume176
DOIs
StatePublished - 2024/07

Keywords

  • 19(10 → 9)abeo-Euphane triterpenoids
  • Meliaceae
  • NO production inhibitory activity
  • Trichilia dregeana

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

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