Mild hyperthermia prior to electroporation increases transfection efficiency in HCT 116, HeLa S3 and SGC 7901 cells

Zheng Li Wei; Ryohei Ogawa; Ichiro Takasaki; Qing Li Zhao; Hua Chuan Zheng; Kanwal Ahmed; Mariame A. Hassan; Takashi Kondo

doi:10.1007/s10529-009-0164-5

Mild hyperthermia prior to electroporation increases transfection efficiency in HCT 116, HeLa S3 and SGC 7901 cells

Zheng Li Wei, Ryohei Ogawa, Ichiro Takasaki, Qing Li Zhao, Hua Chuan Zheng, Kanwal Ahmed, Mariame A. Hassan, Takashi Kondo^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The change in transfection efficiency of electroporation by the combined treatment with mild preheating (40°C for 30 min) was investigated. HCT 116, HeLa S3 and SGC 7901 cells were treated with electroporation in medium containing pBKCMV-Luc plasmid with or without preheating. After 24 h, luciferase activity was increased by 36, 28 and 77%; luciferase mRNA transcription was increased by 45, 50 and 68%; and fluorescein isothiocyanate-dextran accumulation was increased by 9, 35 and 15% in preheated groups, respectively. These results demonstrate that the transfection efficiency was enhanced by mild preheating. The mechanism partially involves increased macromolecular particle accumulation.

Original language	English
Pages (from-to)	367-371
Number of pages	5
Journal	Biotechnology Letters
Volume	32
Issue number	3
DOIs	https://doi.org/10.1007/s10529-009-0164-5
State	Published - 2010/02

Keywords

Efficiency
Electroporation
Mild hyperthermia
Preheating
Transfection

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1007/s10529-009-0164-5

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title = "Mild hyperthermia prior to electroporation increases transfection efficiency in HCT 116, HeLa S3 and SGC 7901 cells",

abstract = "The change in transfection efficiency of electroporation by the combined treatment with mild preheating (40°C for 30 min) was investigated. HCT 116, HeLa S3 and SGC 7901 cells were treated with electroporation in medium containing pBKCMV-Luc plasmid with or without preheating. After 24 h, luciferase activity was increased by 36, 28 and 77%; luciferase mRNA transcription was increased by 45, 50 and 68%; and fluorescein isothiocyanate-dextran accumulation was increased by 9, 35 and 15% in preheated groups, respectively. These results demonstrate that the transfection efficiency was enhanced by mild preheating. The mechanism partially involves increased macromolecular particle accumulation.",

keywords = "Efficiency, Electroporation, Mild hyperthermia, Preheating, Transfection",

author = "Wei, {Zheng Li} and Ryohei Ogawa and Ichiro Takasaki and Zhao, {Qing Li} and Zheng, {Hua Chuan} and Kanwal Ahmed and Hassan, {Mariame A.} and Takashi Kondo",

year = "2010",

month = feb,

doi = "10.1007/s10529-009-0164-5",

language = "英語",

volume = "32",

pages = "367--371",

journal = "Biotechnology Letters",

issn = "0141-5492",

publisher = "Springer Science and Business Media B.V.",

number = "3",

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TY - JOUR

T1 - Mild hyperthermia prior to electroporation increases transfection efficiency in HCT 116, HeLa S3 and SGC 7901 cells

AU - Wei, Zheng Li

AU - Ogawa, Ryohei

AU - Takasaki, Ichiro

AU - Zhao, Qing Li

AU - Zheng, Hua Chuan

AU - Ahmed, Kanwal

AU - Hassan, Mariame A.

AU - Kondo, Takashi

PY - 2010/2

Y1 - 2010/2

N2 - The change in transfection efficiency of electroporation by the combined treatment with mild preheating (40°C for 30 min) was investigated. HCT 116, HeLa S3 and SGC 7901 cells were treated with electroporation in medium containing pBKCMV-Luc plasmid with or without preheating. After 24 h, luciferase activity was increased by 36, 28 and 77%; luciferase mRNA transcription was increased by 45, 50 and 68%; and fluorescein isothiocyanate-dextran accumulation was increased by 9, 35 and 15% in preheated groups, respectively. These results demonstrate that the transfection efficiency was enhanced by mild preheating. The mechanism partially involves increased macromolecular particle accumulation.

AB - The change in transfection efficiency of electroporation by the combined treatment with mild preheating (40°C for 30 min) was investigated. HCT 116, HeLa S3 and SGC 7901 cells were treated with electroporation in medium containing pBKCMV-Luc plasmid with or without preheating. After 24 h, luciferase activity was increased by 36, 28 and 77%; luciferase mRNA transcription was increased by 45, 50 and 68%; and fluorescein isothiocyanate-dextran accumulation was increased by 9, 35 and 15% in preheated groups, respectively. These results demonstrate that the transfection efficiency was enhanced by mild preheating. The mechanism partially involves increased macromolecular particle accumulation.

KW - Efficiency

KW - Electroporation

KW - Mild hyperthermia

KW - Preheating

KW - Transfection

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U2 - 10.1007/s10529-009-0164-5

DO - 10.1007/s10529-009-0164-5

M3 - 学術論文

C2 - 19898781

AN - SCOPUS:77649343001

SN - 0141-5492

VL - 32

SP - 367

EP - 371

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 3

ER -

Mild hyperthermia prior to electroporation increases transfection efficiency in HCT 116, HeLa S3 and SGC 7901 cells

Abstract

Keywords

ASJC Scopus subject areas

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