Ionizing radiation enhances Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL)-induced apoptosis through up-regulations of Death Receptor 4 (DR4) and Death Receptor 5 (DR5) in human osteosarcoma cells

Takeshi Hori; Takashi Kondo; Masahiko Kanamori; Yoshiaki Tabuchi; Ryohei Ogawa; Qing Li Zhao; Kanwal Ahmed; Taketoshi Yasuda; Shoji Seki; Kayo Suzuki; Tomoatsu Kimura

doi:10.1002/jor.21056

Ionizing radiation enhances Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL)-induced apoptosis through up-regulations of Death Receptor 4 (DR4) and Death Receptor 5 (DR5) in human osteosarcoma cells

Takeshi Hori, Takashi Kondo^*, Masahiko Kanamori, Yoshiaki Tabuchi, Ryohei Ogawa, Qing Li Zhao, Kanwal Ahmed, Taketoshi Yasuda, Shoji Seki, Kayo Suzuki, Tomoatsu Kimura

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Despite improvements in chemotherapy and surgery in the treatment of osteosarcoma (OS), satisfactory results are still difficult to achieve. Novel therapeutic modalities need to be developed for osteosarcoma treatment. The combined effects of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and ionizing radiation (IR) on human OS cells were investigated. IR and TRAIL treatment synergistically decreased the cell viability and enhanced apoptosis in OS cell lines. IR pretreatment enhances TRAIL-induced Bid and caspase-3 activations. Decreases in the expression levels of the antiapoptotic proteins c-FLIP and XIAP also associated with apoptosis enhancement. Furthermore, IR pretreatment enhanced DR4 and DR5 expressions at the transcription stage. These results can become the basic lines of evidence for the future treatment of OS using TRAIL with IR.

Original language	English
Pages (from-to)	739-745
Number of pages	7
Journal	Journal of Orthopaedic Research
Volume	28
Issue number	6
DOIs	https://doi.org/10.1002/jor.21056
State	Published - 2010/06

Keywords

Apoptosis
DR5
Ionizing radiation
TRAIL

ASJC Scopus subject areas

Orthopedics and Sports Medicine

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Hori, T., Kondo, T., Kanamori, M., Tabuchi, Y., Ogawa, R., Zhao, Q. L., Ahmed, K., Yasuda, T., Seki, S., Suzuki, K., & Kimura, T. (2010). Ionizing radiation enhances Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL)-induced apoptosis through up-regulations of Death Receptor 4 (DR4) and Death Receptor 5 (DR5) in human osteosarcoma cells. Journal of Orthopaedic Research, 28(6), 739-745. https://doi.org/10.1002/jor.21056

@article{31f3c47c0b13477d9982d64612b58101,

title = "Ionizing radiation enhances Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL)-induced apoptosis through up-regulations of Death Receptor 4 (DR4) and Death Receptor 5 (DR5) in human osteosarcoma cells",

abstract = "Despite improvements in chemotherapy and surgery in the treatment of osteosarcoma (OS), satisfactory results are still difficult to achieve. Novel therapeutic modalities need to be developed for osteosarcoma treatment. The combined effects of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and ionizing radiation (IR) on human OS cells were investigated. IR and TRAIL treatment synergistically decreased the cell viability and enhanced apoptosis in OS cell lines. IR pretreatment enhances TRAIL-induced Bid and caspase-3 activations. Decreases in the expression levels of the antiapoptotic proteins c-FLIP and XIAP also associated with apoptosis enhancement. Furthermore, IR pretreatment enhanced DR4 and DR5 expressions at the transcription stage. These results can become the basic lines of evidence for the future treatment of OS using TRAIL with IR.",

keywords = "Apoptosis, DR5, Ionizing radiation, TRAIL",

author = "Takeshi Hori and Takashi Kondo and Masahiko Kanamori and Yoshiaki Tabuchi and Ryohei Ogawa and Zhao, {Qing Li} and Kanwal Ahmed and Taketoshi Yasuda and Shoji Seki and Kayo Suzuki and Tomoatsu Kimura",

year = "2010",

month = jun,

doi = "10.1002/jor.21056",

language = "英語",

volume = "28",

pages = "739--745",

journal = "Journal of Orthopaedic Research",

issn = "0736-0266",

publisher = "Wiley-Blackwell",

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Hori, T, Kondo, T, Kanamori, M , Tabuchi, Y , Ogawa, R , Zhao, QL, Ahmed, K, Yasuda, T, Seki, S , Suzuki, K & Kimura, T 2010, 'Ionizing radiation enhances Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL)-induced apoptosis through up-regulations of Death Receptor 4 (DR4) and Death Receptor 5 (DR5) in human osteosarcoma cells', Journal of Orthopaedic Research, vol. 28, no. 6, pp. 739-745. https://doi.org/10.1002/jor.21056

Ionizing radiation enhances Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL)-induced apoptosis through up-regulations of Death Receptor 4 (DR4) and Death Receptor 5 (DR5) in human osteosarcoma cells. / Hori, Takeshi; Kondo, Takashi; Kanamori, Masahiko et al.
In: Journal of Orthopaedic Research, Vol. 28, No. 6, 06.2010, p. 739-745.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Ionizing radiation enhances Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL)-induced apoptosis through up-regulations of Death Receptor 4 (DR4) and Death Receptor 5 (DR5) in human osteosarcoma cells

AU - Hori, Takeshi

AU - Kondo, Takashi

AU - Kanamori, Masahiko

AU - Tabuchi, Yoshiaki

AU - Ogawa, Ryohei

AU - Zhao, Qing Li

AU - Ahmed, Kanwal

AU - Yasuda, Taketoshi

AU - Seki, Shoji

AU - Suzuki, Kayo

AU - Kimura, Tomoatsu

PY - 2010/6

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AB - Despite improvements in chemotherapy and surgery in the treatment of osteosarcoma (OS), satisfactory results are still difficult to achieve. Novel therapeutic modalities need to be developed for osteosarcoma treatment. The combined effects of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and ionizing radiation (IR) on human OS cells were investigated. IR and TRAIL treatment synergistically decreased the cell viability and enhanced apoptosis in OS cell lines. IR pretreatment enhances TRAIL-induced Bid and caspase-3 activations. Decreases in the expression levels of the antiapoptotic proteins c-FLIP and XIAP also associated with apoptosis enhancement. Furthermore, IR pretreatment enhanced DR4 and DR5 expressions at the transcription stage. These results can become the basic lines of evidence for the future treatment of OS using TRAIL with IR.

KW - Apoptosis

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