TY - JOUR
T1 - Inhibitory effects of triterpenes isolated from chuling (Polyporus umbellatus FRIES) on free radical-induced lysis of red blood cells
AU - Sekiya, Nobuyasu
AU - Hikiami, Hiroaki
AU - Nakai, Yoichiro
AU - Sakakibara, Iwao
AU - Nozaki, Kazuya
AU - Kouta, Kazufumi
AU - Shimada, Yutaka
AU - Terasawa, Katsutoshi
PY - 2005/5
Y1 - 2005/5
N2 - Chuling, sclerotia of Polyporus umbellatus FRIES, has long been used for urological disorders in traditional medicine. In this study, we demonstrated that Chuling in vitro protects red blood cells from 2,2-azo-bis(2- amidinopropane)dihydrochloride (AAPH)-induced hemolysis. The inhibitory effect was dose-dependent at concentrations of 50 to 1000 μg/ml. Moreover, tests were carried out to identify the main ingredient of Chuling with scavenging effect on free radicals. Triterpene carboxylic acids isolated from the methanol extract of Chuling, namely, polyporusterone A and polyporusterone B, were found to have inhibitory activities against AAPH-induced lysis of red blood cells. The anti-hemolytic effect was significantly stronger in polyporusterone B compared with polyporusterone A. Furthermore, the ingestion of 150 mg of Chuling was associated with a significant increase in free-radical scavenging effect of plasma in rats.
AB - Chuling, sclerotia of Polyporus umbellatus FRIES, has long been used for urological disorders in traditional medicine. In this study, we demonstrated that Chuling in vitro protects red blood cells from 2,2-azo-bis(2- amidinopropane)dihydrochloride (AAPH)-induced hemolysis. The inhibitory effect was dose-dependent at concentrations of 50 to 1000 μg/ml. Moreover, tests were carried out to identify the main ingredient of Chuling with scavenging effect on free radicals. Triterpene carboxylic acids isolated from the methanol extract of Chuling, namely, polyporusterone A and polyporusterone B, were found to have inhibitory activities against AAPH-induced lysis of red blood cells. The anti-hemolytic effect was significantly stronger in polyporusterone B compared with polyporusterone A. Furthermore, the ingestion of 150 mg of Chuling was associated with a significant increase in free-radical scavenging effect of plasma in rats.
KW - Anti-oxidative activity
KW - Chuling
KW - Hemolysis
KW - Polyporus umbellatus
KW - Triterpene
UR - http://www.scopus.com/inward/record.url?scp=19644395374&partnerID=8YFLogxK
U2 - 10.1248/bpb.28.817
DO - 10.1248/bpb.28.817
M3 - 学術論文
C2 - 15863885
AN - SCOPUS:19644395374
SN - 0918-6158
VL - 28
SP - 817
EP - 821
JO - Biological and Pharmaceutical Bulletin
JF - Biological and Pharmaceutical Bulletin
IS - 5
ER -