TY - JOUR
T1 - Inhibitory effects of Stephania tetrandra S. Moore on free radical-induced lysis of rat red blood cells
AU - Sekiya, Nobuyasu
AU - Hikiami, Hiroaki
AU - Yokoyama, Koichi
AU - Kouta, Kazufumi
AU - Sakakibara, Iwao
AU - Shimada, Yutaka
AU - Terasawa, Katsutoshi
PY - 2005/4
Y1 - 2005/4
N2 - Crude preparations of Stephania tetrandra S. MOORE (ST), a traditional herbal medicine, have been used safely for arthritis and silicosis in China. In this study, we demonstrated that ST in vitro protects red blood cells from 2,2-azo-bis (2-amidinopropane) dihydrochloride (AAPH)-induced hemolysis. The inhibitory effect was dose-dependent at concentrations of 10 to 1000 μg/ml. Moreover, tests were carried out to identify the main ingredient of ST that exerts a scavenging effect on free-radicals. Three representative alkaloids, tetrandrine, fangchinoline, and cyclanoline, isolated from ST, were found to have inhibitory activities against AAPH-induced lysis of red blood cells (RBC). Furthermore, the ingestion of 200 mg of ST extract was associated with a significant increase in free-radical scavenging effect of plasma in rats. These results suggest that ST as antioxidant inhibits AAPH-induced hemolysis of RBC both in vitro and in vivo.
AB - Crude preparations of Stephania tetrandra S. MOORE (ST), a traditional herbal medicine, have been used safely for arthritis and silicosis in China. In this study, we demonstrated that ST in vitro protects red blood cells from 2,2-azo-bis (2-amidinopropane) dihydrochloride (AAPH)-induced hemolysis. The inhibitory effect was dose-dependent at concentrations of 10 to 1000 μg/ml. Moreover, tests were carried out to identify the main ingredient of ST that exerts a scavenging effect on free-radicals. Three representative alkaloids, tetrandrine, fangchinoline, and cyclanoline, isolated from ST, were found to have inhibitory activities against AAPH-induced lysis of red blood cells (RBC). Furthermore, the ingestion of 200 mg of ST extract was associated with a significant increase in free-radical scavenging effect of plasma in rats. These results suggest that ST as antioxidant inhibits AAPH-induced hemolysis of RBC both in vitro and in vivo.
KW - Antioxidative effect
KW - Reactive oxygen specie
KW - Stephania tetrandra
UR - http://www.scopus.com/inward/record.url?scp=21144436679&partnerID=8YFLogxK
U2 - 10.1248/bpb.28.667
DO - 10.1248/bpb.28.667
M3 - 学術論文
C2 - 15802807
AN - SCOPUS:21144436679
SN - 0918-6158
VL - 28
SP - 667
EP - 670
JO - Biological and Pharmaceutical Bulletin
JF - Biological and Pharmaceutical Bulletin
IS - 4
ER -