TY - JOUR
T1 - Genetic response to low-intensity ultrasound on mouse ST2 bone marrow stromal cells
AU - Tabuchi, Yoshiaki
AU - Hasegawa, Hideyuki
AU - Suzuki, Nobuo
AU - Furusawa, Yukihiro
AU - Hirano, Tetsushi
AU - Nagaoka, Ryo
AU - Hirayama, Jun
AU - Hoshi, Nobuhiko
AU - Mochizuki, Takashi
N1 - Publisher Copyright:
© This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) License.
PY - 2020/12
Y1 - 2020/12
N2 - Although low-intensity ultrasound (LIUS) is a clinically established procedure, the early cellular effect of LIUS on a genetic level has not yet been studied. The current study investigated the early response genes elicited by LIUS in bone marrow stromal cells (BMSCs) using global-scale microarrays and computational gene expression analysis tools. Mouse ST2 BMSCs were treated with LIUS [ISATA, 25 mW/cm2 for 20 min with a frequency of 1.11 MHz in a pulsed-wave mode (0.2-s burst sine waves repeated at 1 kHz)], then cultured for 0.5, 1 and 3 h at 37 C. The time course of changes in gene expression was evaluated using GeneChip® high-density oligonucleotide microarrays and Ingenuity® Pathway Analysis tools. The results were verified by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). A single exposure of LIUS did not affect cell morphology, cell growth or alkaline phosphatase activity. However, 61 upregulated and 103 downregulated genes were identified from 0.5 to 3 h after LIUS treatment. Two significant gene networks, labeled E and H, were identified from the upregulated genes, while a third network, labeled T, was identified from the downregulated genes. Gene network E or H containing the immediate-early genes FBJ osteosarcoma oncogene and early growth response 1 or the heat shock proteins heat shock protein 1a/b was associated mainly with the biological functions of bone physiology and protein folding or apoptosis, respectively. Gene network T containing transcription factors fos-like antigen 1 and serum response factor was also associated with the biological functions of the gene expression. RT-qPCR indicated that the expression of several genes in the gene networks E and H were elevated in LIUS-treated cells. LIUS was demonstrated to induce gene expression after short application in mouse ST2 BMSCs. The results of the present study provide a basis for the elucidation of the detailed molecular mechanisms underlying the cellular effects of LIUS.
AB - Although low-intensity ultrasound (LIUS) is a clinically established procedure, the early cellular effect of LIUS on a genetic level has not yet been studied. The current study investigated the early response genes elicited by LIUS in bone marrow stromal cells (BMSCs) using global-scale microarrays and computational gene expression analysis tools. Mouse ST2 BMSCs were treated with LIUS [ISATA, 25 mW/cm2 for 20 min with a frequency of 1.11 MHz in a pulsed-wave mode (0.2-s burst sine waves repeated at 1 kHz)], then cultured for 0.5, 1 and 3 h at 37 C. The time course of changes in gene expression was evaluated using GeneChip® high-density oligonucleotide microarrays and Ingenuity® Pathway Analysis tools. The results were verified by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). A single exposure of LIUS did not affect cell morphology, cell growth or alkaline phosphatase activity. However, 61 upregulated and 103 downregulated genes were identified from 0.5 to 3 h after LIUS treatment. Two significant gene networks, labeled E and H, were identified from the upregulated genes, while a third network, labeled T, was identified from the downregulated genes. Gene network E or H containing the immediate-early genes FBJ osteosarcoma oncogene and early growth response 1 or the heat shock proteins heat shock protein 1a/b was associated mainly with the biological functions of bone physiology and protein folding or apoptosis, respectively. Gene network T containing transcription factors fos-like antigen 1 and serum response factor was also associated with the biological functions of the gene expression. RT-qPCR indicated that the expression of several genes in the gene networks E and H were elevated in LIUS-treated cells. LIUS was demonstrated to induce gene expression after short application in mouse ST2 BMSCs. The results of the present study provide a basis for the elucidation of the detailed molecular mechanisms underlying the cellular effects of LIUS.
KW - Gene expression
KW - Heat shock proteins
KW - Immediate-early genes
KW - Low-intensity ultrasound
KW - ST2 bone marrow stromal cell
UR - http://www.scopus.com/inward/record.url?scp=85099228438&partnerID=8YFLogxK
U2 - 10.3892/MMR.2020.11812
DO - 10.3892/MMR.2020.11812
M3 - 学術論文
C2 - 33398373
AN - SCOPUS:85099228438
SN - 1791-2997
VL - 23
JO - Molecular Medicine Reports
JF - Molecular Medicine Reports
IS - 3
M1 - 11812
ER -
