Fabrication of Cardiac Constructs Using Bio-3D Printer

Kenichi Arai, Daiki Murata, Shoko Takao, Koichi Nakayama*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

5 Scopus citations

Abstract

The fabrication of three-dimensional (3D) cardiac tissue using human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) is useful not only for regenerative medicine, but also for drug discovery. Here, we report a bio-3D printer that can fabricate tubular cardiac constructs using only human iPSC-CMs. Protocols to evaluate the contractile force and response to electrical stimulation in the cardiac constructs are described. We confirmed that the constructs can be applied for transplantation or drug response testing. In the near future, we expect that the constructs will be used as alternatives for heart transplantation and in animal experiments for new drug development.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages53-63
Number of pages11
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2320
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Bio-3D printer
  • Biofabrication
  • Cardiac construct
  • Human induced pluripotent stem cell-derived cardiomyocytes
  • Scaffold-free

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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