TY - JOUR
T1 - Evaluation of a novel urinary antigen test kit for diagnosing Legionella pneumonia
AU - Ito, Akihiro
AU - Yamamoto, Yoshihiro
AU - Ishii, Yoshikazu
AU - Okazaki, Akihito
AU - Ishiura, Yoshihisa
AU - Kawagishi, Yukio
AU - Takiguchi, Yasuo
AU - Kishi, Kazuma
AU - Taguchi, Yoshio
AU - Shinzato, Takashi
AU - Okochi, Yasumi
AU - Hayashi, Ryuji
AU - Nakamori, Yoshitaka
AU - Kichikawa, Yoshiko
AU - Murata, Kengo
AU - Takeda, Hiroaki
AU - Higa, Futoshi
AU - Miyara, Takayuki
AU - Saito, Keisuke
AU - Ishikawa, Takeo
AU - Ishida, Tadashi
AU - Tateda, Kazuhiro
N1 - Publisher Copyright:
© 2020 The Authors
PY - 2021/2
Y1 - 2021/2
N2 - Objectives: The aim of this study was to evaluate the diagnostic utility of a novel test kit that could theoretically detect all serogroups of Legionella pneumophila for diagnosing Legionella pneumonia, in comparison with existing kits. Methods: This study was conducted in 16 hospitals in Japan from April 2016 to December 2018. Three urinary antigen test kits were used: the novel kit (LAC-116), BinaxNOW Legionella (Binax), and Q-line Kyokutou Legionella (Q-line). In addition, sputum culture and nucleic acid detection tests and serum antibody tests were performed where possible. The diagnostic accuracy and correlations of the novel kit with the two existing kits were analyzed. Results: In total, 56 patients were diagnosed with Legionella pneumonia. The sensitivities of LAC-116, Binax, and Q-line were 79%, 84%, and 71%, respectively. The overall match rate between LAC-116 and Binax was 96.8% and between LAC-116 and Q-line was 96.4%. One patient had L. pneumophila serogroup 2, and only LAC-116 showed a positive result, whereas Binax and Q-line did not. Conclusions: The novel Legionella urinary antigen test kit was useful for diagnosing Legionella pneumonia. In addition, it could detect Legionella pneumonia caused by non-L. pneumophila serogroup 1.
AB - Objectives: The aim of this study was to evaluate the diagnostic utility of a novel test kit that could theoretically detect all serogroups of Legionella pneumophila for diagnosing Legionella pneumonia, in comparison with existing kits. Methods: This study was conducted in 16 hospitals in Japan from April 2016 to December 2018. Three urinary antigen test kits were used: the novel kit (LAC-116), BinaxNOW Legionella (Binax), and Q-line Kyokutou Legionella (Q-line). In addition, sputum culture and nucleic acid detection tests and serum antibody tests were performed where possible. The diagnostic accuracy and correlations of the novel kit with the two existing kits were analyzed. Results: In total, 56 patients were diagnosed with Legionella pneumonia. The sensitivities of LAC-116, Binax, and Q-line were 79%, 84%, and 71%, respectively. The overall match rate between LAC-116 and Binax was 96.8% and between LAC-116 and Q-line was 96.4%. One patient had L. pneumophila serogroup 2, and only LAC-116 showed a positive result, whereas Binax and Q-line did not. Conclusions: The novel Legionella urinary antigen test kit was useful for diagnosing Legionella pneumonia. In addition, it could detect Legionella pneumonia caused by non-L. pneumophila serogroup 1.
KW - Diagnosis
KW - Immunoassay
KW - Legionella pneumophila
KW - Pneumonia
KW - Urinary antigen test
UR - http://www.scopus.com/inward/record.url?scp=85097900635&partnerID=8YFLogxK
U2 - 10.1016/j.ijid.2020.10.106
DO - 10.1016/j.ijid.2020.10.106
M3 - 学術論文
C2 - 33176204
AN - SCOPUS:85097900635
SN - 1201-9712
VL - 103
SP - 42
EP - 47
JO - International Journal of Infectious Diseases
JF - International Journal of Infectious Diseases
ER -