Elucidation of the neurological effects of clothianidin exposure at the no-observed-adverse-effect level (NOAEL) using two-photon microscopy in vivo imaging

Misaki Nishi; Shouta Sugio; Tetsushi Hirano; Daisuke Kato; Hiroaki Wake; Asuka Shoda; Midori Murata; Yoshinori Ikenaka; Yoshiaki Tabuchi; Youhei Mantani; Toshifumi Yokoyama; Nobuhiko Hoshi

doi:10.1292/jvms.22-0013

Elucidation of the neurological effects of clothianidin exposure at the no-observed-adverse-effect level (NOAEL) using two-photon microscopy in vivo imaging

Misaki Nishi, Shouta Sugio, Tetsushi Hirano, Daisuke Kato, Hiroaki Wake, Asuka Shoda, Midori Murata, Yoshinori Ikenaka, Yoshiaki Tabuchi, Youhei Mantani, Toshifumi Yokoyama, Nobuhiko Hoshi^*

^*Corresponding author for this work

Division of Drug and Structural Research, Life Science Research Center, Organization for Promotion of Research
Division of Molecular Genetics Research, Life Science Research Center, Organization for Promotion of Research

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Neonicotinoid pesticides (NNs) cause behavioral abnormalities in mammals, raising concerns about their effects on neural circuit activity. We herein examined the neurological effectof the NN clothianidin (CLO) by in vivo Ca²⁺ imaging using two-photon microscopy. Mice were fed the no-observed-adverse-effect-level (NOAEL) dose of CLO for 2 weeks and their neuronal activity in the primary somatosensory cortex (S1) was observed weekly for 2 weeks. CLO exposure caused a sustained influx of Ca²⁺ in neurons in the S1 2/3 layers, indicating hyperactivation of neurons. In addition, microarray gene expression analysis suggested the induction of neuroinflammation and changes in synaptic activity. These results demonstrate that exposure to the NOAEL dose of CLO can overactivate neurons and disrupt neuronal homeostasis.

Original language	English
Pages (from-to)	585-592
Number of pages	8
Journal	Journal of Veterinary Medical Science
Volume	84
Issue number	4
DOIs	https://doi.org/10.1292/jvms.22-0013
State	Published - 2022

Keywords

clothianidin
neonicotinoid
neurotransmission
two-photon calcium imaging

ASJC Scopus subject areas

General Veterinary

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10.1292/jvms.22-0013

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Nishi, M., Sugio, S., Hirano, T., Kato, D., Wake, H., Shoda, A., Murata, M., Ikenaka, Y., Tabuchi, Y., Mantani, Y., Yokoyama, T., & Hoshi, N. (2022). Elucidation of the neurological effects of clothianidin exposure at the no-observed-adverse-effect level (NOAEL) using two-photon microscopy in vivo imaging. Journal of Veterinary Medical Science, 84(4), 585-592. https://doi.org/10.1292/jvms.22-0013

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title = "Elucidation of the neurological effects of clothianidin exposure at the no-observed-adverse-effect level (NOAEL) using two-photon microscopy in vivo imaging",

abstract = "Neonicotinoid pesticides (NNs) cause behavioral abnormalities in mammals, raising concerns about their effects on neural circuit activity. We herein examined the neurological effectof the NN clothianidin (CLO) by in vivo Ca2+ imaging using two-photon microscopy. Mice were fed the no-observed-adverse-effect-level (NOAEL) dose of CLO for 2 weeks and their neuronal activity in the primary somatosensory cortex (S1) was observed weekly for 2 weeks. CLO exposure caused a sustained influx of Ca2+ in neurons in the S1 2/3 layers, indicating hyperactivation of neurons. In addition, microarray gene expression analysis suggested the induction of neuroinflammation and changes in synaptic activity. These results demonstrate that exposure to the NOAEL dose of CLO can overactivate neurons and disrupt neuronal homeostasis.",

keywords = "clothianidin, neonicotinoid, neurotransmission, two-photon calcium imaging",

author = "Misaki Nishi and Shouta Sugio and Tetsushi Hirano and Daisuke Kato and Hiroaki Wake and Asuka Shoda and Midori Murata and Yoshinori Ikenaka and Yoshiaki Tabuchi and Youhei Mantani and Toshifumi Yokoyama and Nobuhiko Hoshi",

note = "Publisher Copyright: {\textcopyright} 2022 The Japanese Society of Veterinary Science.",

year = "2022",

doi = "10.1292/jvms.22-0013",

language = "英語",

volume = "84",

pages = "585--592",

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Nishi, M, Sugio, S, Hirano, T, Kato, D, Wake, H, Shoda, A, Murata, M, Ikenaka, Y, Tabuchi, Y, Mantani, Y, Yokoyama, T & Hoshi, N 2022, 'Elucidation of the neurological effects of clothianidin exposure at the no-observed-adverse-effect level (NOAEL) using two-photon microscopy in vivo imaging', Journal of Veterinary Medical Science, vol. 84, no. 4, pp. 585-592. https://doi.org/10.1292/jvms.22-0013

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T1 - Elucidation of the neurological effects of clothianidin exposure at the no-observed-adverse-effect level (NOAEL) using two-photon microscopy in vivo imaging

AU - Nishi, Misaki

AU - Sugio, Shouta

AU - Hirano, Tetsushi

AU - Kato, Daisuke

AU - Wake, Hiroaki

AU - Shoda, Asuka

AU - Murata, Midori

AU - Ikenaka, Yoshinori

AU - Tabuchi, Yoshiaki

AU - Mantani, Youhei

AU - Yokoyama, Toshifumi

AU - Hoshi, Nobuhiko

PY - 2022

Y1 - 2022

N2 - Neonicotinoid pesticides (NNs) cause behavioral abnormalities in mammals, raising concerns about their effects on neural circuit activity. We herein examined the neurological effectof the NN clothianidin (CLO) by in vivo Ca2+ imaging using two-photon microscopy. Mice were fed the no-observed-adverse-effect-level (NOAEL) dose of CLO for 2 weeks and their neuronal activity in the primary somatosensory cortex (S1) was observed weekly for 2 weeks. CLO exposure caused a sustained influx of Ca2+ in neurons in the S1 2/3 layers, indicating hyperactivation of neurons. In addition, microarray gene expression analysis suggested the induction of neuroinflammation and changes in synaptic activity. These results demonstrate that exposure to the NOAEL dose of CLO can overactivate neurons and disrupt neuronal homeostasis.

AB - Neonicotinoid pesticides (NNs) cause behavioral abnormalities in mammals, raising concerns about their effects on neural circuit activity. We herein examined the neurological effectof the NN clothianidin (CLO) by in vivo Ca2+ imaging using two-photon microscopy. Mice were fed the no-observed-adverse-effect-level (NOAEL) dose of CLO for 2 weeks and their neuronal activity in the primary somatosensory cortex (S1) was observed weekly for 2 weeks. CLO exposure caused a sustained influx of Ca2+ in neurons in the S1 2/3 layers, indicating hyperactivation of neurons. In addition, microarray gene expression analysis suggested the induction of neuroinflammation and changes in synaptic activity. These results demonstrate that exposure to the NOAEL dose of CLO can overactivate neurons and disrupt neuronal homeostasis.

KW - clothianidin

KW - neonicotinoid

KW - neurotransmission

KW - two-photon calcium imaging

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U2 - 10.1292/jvms.22-0013

DO - 10.1292/jvms.22-0013

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C2 - 35264496

AN - SCOPUS:85128427840

SN - 0916-7250

VL - 84

SP - 585

EP - 592

JO - Journal of Veterinary Medical Science

JF - Journal of Veterinary Medical Science

IS - 4

ER -

Elucidation of the neurological effects of clothianidin exposure at the no-observed-adverse-effect level (NOAEL) using two-photon microscopy in vivo imaging

Abstract

Keywords

ASJC Scopus subject areas

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