Effects of microbubbles on ultrasound-mediated gene transfer in human prostate cancer PC3 cells: Comparison among Levovist, YM454, and MRX-815H

Akihiko Watanabe; Remon Otake; Tetsuo Nozaki; Akihiro Morii; Ryohei Ogawa; Shinichi Fujimoto; Shinobu Nakamura; Hideki Fuse; Takashi Kondo

doi:10.1016/j.canlet.2008.02.004

Effects of microbubbles on ultrasound-mediated gene transfer in human prostate cancer PC3 cells: Comparison among Levovist, YM454, and MRX-815H

Akihiko Watanabe, Remon Otake, Tetsuo Nozaki, Akihiro Morii, Ryohei Ogawa^*, Shinichi Fujimoto, Shinobu Nakamura, Hideki Fuse, Takashi Kondo

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

We compared the enhancement effects of three different echo contrast agents (ECAs); Levovist, YM454, and MRX-815H as artificial microbubbles on ultrasound mediated gene transfection (USMGT) with 1 MHz ultrasound at 0.2 MPa using a luciferase expression vector in PC3 cells and elucidated the mechanisms of differences of USMGT facilitation by these ECAs. At a concentration of each ECA that induced iso-survival, ECAs with lipid shell (YM454 and MRX-815H) facilitated USMGT higher than those without shell (Levovist), and the order of the ECAs facilitating free radical formation by sonication was; YM454 > MRX-815H > Levovist. These results suggested that the lipid shell type ECAs facilitated gene transfer higher than that by the non-shell type ECA.

Original language	English
Pages (from-to)	107-112
Number of pages	6
Journal	Cancer Letters
Volume	265
Issue number	1
DOIs	https://doi.org/10.1016/j.canlet.2008.02.004
State	Published - 2008/06/28

Keywords

Echo contrast agent
Microbubble
Sonication
Transfection
Ultrasonic

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.canlet.2008.02.004

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title = "Effects of microbubbles on ultrasound-mediated gene transfer in human prostate cancer PC3 cells: Comparison among Levovist, YM454, and MRX-815H",

abstract = "We compared the enhancement effects of three different echo contrast agents (ECAs); Levovist, YM454, and MRX-815H as artificial microbubbles on ultrasound mediated gene transfection (USMGT) with 1 MHz ultrasound at 0.2 MPa using a luciferase expression vector in PC3 cells and elucidated the mechanisms of differences of USMGT facilitation by these ECAs. At a concentration of each ECA that induced iso-survival, ECAs with lipid shell (YM454 and MRX-815H) facilitated USMGT higher than those without shell (Levovist), and the order of the ECAs facilitating free radical formation by sonication was; YM454 > MRX-815H > Levovist. These results suggested that the lipid shell type ECAs facilitated gene transfer higher than that by the non-shell type ECA.",

keywords = "Echo contrast agent, Microbubble, Sonication, Transfection, Ultrasonic",

author = "Akihiko Watanabe and Remon Otake and Tetsuo Nozaki and Akihiro Morii and Ryohei Ogawa and Shinichi Fujimoto and Shinobu Nakamura and Hideki Fuse and Takashi Kondo",

year = "2008",

month = jun,

day = "28",

doi = "10.1016/j.canlet.2008.02.004",

language = "英語",

volume = "265",

pages = "107--112",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "1",

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TY - JOUR

T1 - Effects of microbubbles on ultrasound-mediated gene transfer in human prostate cancer PC3 cells

T2 - Comparison among Levovist, YM454, and MRX-815H

AU - Watanabe, Akihiko

AU - Otake, Remon

AU - Nozaki, Tetsuo

AU - Morii, Akihiro

AU - Ogawa, Ryohei

AU - Fujimoto, Shinichi

AU - Nakamura, Shinobu

AU - Fuse, Hideki

AU - Kondo, Takashi

PY - 2008/6/28

Y1 - 2008/6/28

N2 - We compared the enhancement effects of three different echo contrast agents (ECAs); Levovist, YM454, and MRX-815H as artificial microbubbles on ultrasound mediated gene transfection (USMGT) with 1 MHz ultrasound at 0.2 MPa using a luciferase expression vector in PC3 cells and elucidated the mechanisms of differences of USMGT facilitation by these ECAs. At a concentration of each ECA that induced iso-survival, ECAs with lipid shell (YM454 and MRX-815H) facilitated USMGT higher than those without shell (Levovist), and the order of the ECAs facilitating free radical formation by sonication was; YM454 > MRX-815H > Levovist. These results suggested that the lipid shell type ECAs facilitated gene transfer higher than that by the non-shell type ECA.

AB - We compared the enhancement effects of three different echo contrast agents (ECAs); Levovist, YM454, and MRX-815H as artificial microbubbles on ultrasound mediated gene transfection (USMGT) with 1 MHz ultrasound at 0.2 MPa using a luciferase expression vector in PC3 cells and elucidated the mechanisms of differences of USMGT facilitation by these ECAs. At a concentration of each ECA that induced iso-survival, ECAs with lipid shell (YM454 and MRX-815H) facilitated USMGT higher than those without shell (Levovist), and the order of the ECAs facilitating free radical formation by sonication was; YM454 > MRX-815H > Levovist. These results suggested that the lipid shell type ECAs facilitated gene transfer higher than that by the non-shell type ECA.

KW - Echo contrast agent

KW - Microbubble

KW - Sonication

KW - Transfection

KW - Ultrasonic

UR - http://www.scopus.com/inward/record.url?scp=43449136747&partnerID=8YFLogxK

U2 - 10.1016/j.canlet.2008.02.004

DO - 10.1016/j.canlet.2008.02.004

M3 - 学術論文

C2 - 18329166

AN - SCOPUS:43449136747

SN - 0304-3835

VL - 265

SP - 107

EP - 112

JO - Cancer Letters

JF - Cancer Letters

IS - 1

ER -

Effects of microbubbles on ultrasound-mediated gene transfer in human prostate cancer PC3 cells: Comparison among Levovist, YM454, and MRX-815H

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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