Abstract
The mechanism by which the neonicotinoid pesticide clothianidin (CLO) disrupts the intestinal microbiota of experimental animals is unknown. We focused on α-defensins, which are regulators of the intestinal microbiota. Subchronic exposure to CLO induced dysbiosis and reduced short-chain fatty acid–producing bacteria in the intestinal microbiota of mice. Levels of cryptdin-1 (Crp1, a major α-defensin in mice) in feces and cecal contents were lower in the CLO-exposed groups than in control. In Crp1 immunostaining, Paneth cells in the jejunum and ileum of the no-observed-adverse-effect-level CLO-exposed group showed a stronger positive signal than control, likely due to the suppression of Crp1 release. Our results showed that CLO exposure suppresses α-defensin secretion from Paneth cells as part of the mechanism underlying CLO-induced dysbiosis.
Original language | English |
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Pages (from-to) | 277-284 |
Number of pages | 8 |
Journal | Journal of Veterinary Medical Science |
Volume | 86 |
Issue number | 3 |
DOIs | |
State | Published - 2024 |
Keywords
- clothianidin
- dysbiosis
- microbiota
- neonicotinoid
- α-defensin
ASJC Scopus subject areas
- General Veterinary