Effects of Eppikahangeto, a Kampo formula, and Ephedrae herba against citric acid-induced laryngeal cough in guinea pigs

Kiyoshi Minamizawa, Hirozo Goto*, Yutaka Shimada, Katsutoshi Terasawa, Akira Haji

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

To evaluate the efficacy of three common antitussive Kampo formulas, eppikahangeto (EPP), bakumondoto (BAK), and shoseiryutogomakyokansekito (SGM), a new cough model of guinea pig was used, which could specifically induce a laryngeal cough by microinjection of citric acid solution into the larynx. Kampo extract was dissolved in water and the animals were given access ad libitum for 3 days, and then the number of coughs during 10 min was counted. EPP extract decreased the number of coughs dose-dependently (0.3% extract, -22.9 ± 6.6%, P<0.01; 1.0% extract, -32.4 ± 5.5%, P<0.01). BAK extract and SGM extract had no significant effect. Intraperitoneal injection of codeine (60 mg/kg) also decreased the number of coughs (-36.1 ± 9.1%, P<0.05). Furthermore, Ephedrae herba (EH) extract reduced the number of coughs (-18.3 ± 6.0%, P<0.05), but the extract of EPP without EH did not. These results suggest that EPP has an antitussive effect against laryngeally-induced cough in guinea pigs, and the crucial herbal medicine is EH.

Original languageEnglish
Pages (from-to)118-125
Number of pages8
JournalJournal of Pharmacological Sciences
Volume101
Issue number2
DOIs
StatePublished - 2006

Keywords

  • Antitussive effect
  • Ephedrae herba
  • Eppikahangeto
  • Kampo medicine
  • Laryngeal cough

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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