Discrepant Diagnostic Results of Nested Polymerase Chain Reaction-based Genotyping in a Patient with Hepatitis C Virus and Human Immunodeficiency Virus Coinfection

Masami Minemura; Kazuto Tajiri; Yuka Hayashi; Naoki Takahashi; Kasumi Watanabe; Tatsuyuki Hanaoka; Yasuhiro Araki; Kosuke Takahashi; Terumi Takahara; Shigeyuki Kojima; Ichiro Yasuda

doi:10.2169/internalmedicine.7132-21

Discrepant Diagnostic Results of Nested Polymerase Chain Reaction-based Genotyping in a Patient with Hepatitis C Virus and Human Immunodeficiency Virus Coinfection

Masami Minemura^*, Kazuto Tajiri, Yuka Hayashi, Naoki Takahashi, Kasumi Watanabe, Tatsuyuki Hanaoka, Yasuhiro Araki, Kosuke Takahashi, Terumi Takahara, Shigeyuki Kojima, Ichiro Yasuda

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Accurate genotyping is important to improve the treatment of hepatitis C virus (HCV) infection. We herein report a 44-year-old Japanese man with hemophilia A and coinfection of HCV and human immunodeficiency virus (HIV) who was diagnosed with HCV genotype 4 by direct sequencing. Two genotyping tests based on the nested polymerase chain reaction method that we used misdiagnosed his genotype as 2b and 1b. Although several HCV genotyping tests are available in Japan, it is important to recognize that some cannot detect genotype 4. Care should be taken when genotyping HCV patients who have received non-heated coagulation factor preparations or were infected abroad.

Original language	English
Pages (from-to)	3239-3243
Number of pages	5
Journal	Internal Medicine
Volume	60
Issue number	20
DOIs	https://doi.org/10.2169/internalmedicine.7132-21
State	Published - 2021

Keywords

Direct-acting antiviral agent
Genotype 4
Genotyping
Hepatitis C virus

ASJC Scopus subject areas

Internal Medicine

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10.2169/internalmedicine.7132-21

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Minemura, M., Tajiri, K., Hayashi, Y., Takahashi, N., Watanabe, K., Hanaoka, T., Araki, Y., Takahashi, K., Takahara, T., Kojima, S., & Yasuda, I. (2021). Discrepant Diagnostic Results of Nested Polymerase Chain Reaction-based Genotyping in a Patient with Hepatitis C Virus and Human Immunodeficiency Virus Coinfection. Internal Medicine, 60(20), 3239-3243. https://doi.org/10.2169/internalmedicine.7132-21

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title = "Discrepant Diagnostic Results of Nested Polymerase Chain Reaction-based Genotyping in a Patient with Hepatitis C Virus and Human Immunodeficiency Virus Coinfection",

abstract = "Accurate genotyping is important to improve the treatment of hepatitis C virus (HCV) infection. We herein report a 44-year-old Japanese man with hemophilia A and coinfection of HCV and human immunodeficiency virus (HIV) who was diagnosed with HCV genotype 4 by direct sequencing. Two genotyping tests based on the nested polymerase chain reaction method that we used misdiagnosed his genotype as 2b and 1b. Although several HCV genotyping tests are available in Japan, it is important to recognize that some cannot detect genotype 4. Care should be taken when genotyping HCV patients who have received non-heated coagulation factor preparations or were infected abroad.",

keywords = "Direct-acting antiviral agent, Genotype 4, Genotyping, Hepatitis C virus",

author = "Masami Minemura and Kazuto Tajiri and Yuka Hayashi and Naoki Takahashi and Kasumi Watanabe and Tatsuyuki Hanaoka and Yasuhiro Araki and Kosuke Takahashi and Terumi Takahara and Shigeyuki Kojima and Ichiro Yasuda",

note = "Publisher Copyright: {\textcopyright} 2021 The Japanese Society of Internal Medicine",

year = "2021",

doi = "10.2169/internalmedicine.7132-21",

language = "英語",

volume = "60",

pages = "3239--3243",

journal = "Internal Medicine",

issn = "0918-2918",

publisher = "Japanese Society of Internal Medicine",

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Minemura, M , Tajiri, K , Hayashi, Y, Takahashi, N, Watanabe, K, Hanaoka, T, Araki, Y, Takahashi, K, Takahara, T, Kojima, S & Yasuda, I 2021, 'Discrepant Diagnostic Results of Nested Polymerase Chain Reaction-based Genotyping in a Patient with Hepatitis C Virus and Human Immunodeficiency Virus Coinfection', Internal Medicine, vol. 60, no. 20, pp. 3239-3243. https://doi.org/10.2169/internalmedicine.7132-21

TY - JOUR

T1 - Discrepant Diagnostic Results of Nested Polymerase Chain Reaction-based Genotyping in a Patient with Hepatitis C Virus and Human Immunodeficiency Virus Coinfection

AU - Minemura, Masami

AU - Tajiri, Kazuto

AU - Hayashi, Yuka

AU - Takahashi, Naoki

AU - Watanabe, Kasumi

AU - Hanaoka, Tatsuyuki

AU - Araki, Yasuhiro

AU - Takahashi, Kosuke

AU - Takahara, Terumi

AU - Kojima, Shigeyuki

AU - Yasuda, Ichiro

PY - 2021

Y1 - 2021

N2 - Accurate genotyping is important to improve the treatment of hepatitis C virus (HCV) infection. We herein report a 44-year-old Japanese man with hemophilia A and coinfection of HCV and human immunodeficiency virus (HIV) who was diagnosed with HCV genotype 4 by direct sequencing. Two genotyping tests based on the nested polymerase chain reaction method that we used misdiagnosed his genotype as 2b and 1b. Although several HCV genotyping tests are available in Japan, it is important to recognize that some cannot detect genotype 4. Care should be taken when genotyping HCV patients who have received non-heated coagulation factor preparations or were infected abroad.

AB - Accurate genotyping is important to improve the treatment of hepatitis C virus (HCV) infection. We herein report a 44-year-old Japanese man with hemophilia A and coinfection of HCV and human immunodeficiency virus (HIV) who was diagnosed with HCV genotype 4 by direct sequencing. Two genotyping tests based on the nested polymerase chain reaction method that we used misdiagnosed his genotype as 2b and 1b. Although several HCV genotyping tests are available in Japan, it is important to recognize that some cannot detect genotype 4. Care should be taken when genotyping HCV patients who have received non-heated coagulation factor preparations or were infected abroad.

KW - Direct-acting antiviral agent

KW - Genotype 4

KW - Genotyping

KW - Hepatitis C virus

UR - http://www.scopus.com/inward/record.url?scp=85117182747&partnerID=8YFLogxK

U2 - 10.2169/internalmedicine.7132-21

DO - 10.2169/internalmedicine.7132-21

M3 - 学術論文

C2 - 33896867

AN - SCOPUS:85117182747

SN - 0918-2918

VL - 60

SP - 3239

EP - 3243

JO - Internal Medicine

JF - Internal Medicine

IS - 20

ER -

Discrepant Diagnostic Results of Nested Polymerase Chain Reaction-based Genotyping in a Patient with Hepatitis C Virus and Human Immunodeficiency Virus Coinfection

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Keywords

ASJC Scopus subject areas

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