Dietary docosahexaenoic acid but not eicosapentaenoic acid suppresses lipopolysaccharide-induced interleukin-1β mRNA induction in mouse spleen leukocytes

S. Watanabe*, K. Katagiri, K. Onozaki, N. Hata, Y. Misawa, T. Hamazaki, O. Okuyama

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Mice were fed a diet supplemented either with beef tallow (BT), BT plus ethyl eicosapentaenoate (EPA) or BT plus ethyl docosahexaenoate (DHA) for 9 weeks. EPA and DHA supplementation increased the content of the respective fatty acid in spleen leukocyte lipids, which was associated with the reduction in the arachidonate content. IL-1β mRNA induction upon lipopolysaccharide (LPS) stimulation in spleen leukocytes in the DHA diet group was significantly lower than in the BT diet group, but the EPA diet was without any significant effect. The amount of prostaglandin E2 (PGE2) released from LPS-stimulated spleen leukocytes was significantly lower in both the EPA and DHA groups than in the BT group. Thus, dietary EPA and DHA inhibited arachidonate metabolism similarly but had different effects on IL-1β mRNA induction in mouse spleen leukocytes. (C) 2000 Harcourt Publishers Ltd.

Original languageEnglish
Pages (from-to)147-152
Number of pages6
JournalProstaglandins Leukotrienes and Essential Fatty Acids
Volume62
Issue number3
DOIs
StatePublished - 2000/03

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

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