TY - JOUR
T1 - CDKN2A, CDK1, and CCNE1 overexpression in sebaceous gland carcinoma of eyelid
AU - Yunoki, Tatsuya
AU - Hirano, Tetsushi
AU - Tabuchi, Yoshiaki
AU - Furusawa, Yukihiro
AU - Torigoe, Misako
AU - Nakajima, Takahiko
AU - Imura, Johji
AU - Hayashi, Atsushi
N1 - Publisher Copyright:
© 2019, Springer Nature B.V.
PY - 2020/2/1
Y1 - 2020/2/1
N2 - Purpose: To investigate the overexpression of genes in sebaceous gland carcinoma (SGC) of the eyelid compared to sebaceous adenoma of the eyelid in order to elucidate the molecular mechanism underlying pathogenesis. Methods: We performed histopathological examination of eyelid tissues surgically removed from four patients diagnosed with SGC (cases 1–3) and sebaceous adenoma (case 4) of the eyelid. Next, we performed global gene expression analysis of surgical tissue samples using a GeneChip® system and the Ingenuity Pathways Knowledge Base. The results of the GeneChip® analysis were explored with quantitative real-time polymerase chain reaction (qRT-PCR) analysis. Results: In the SGC samples, we found that 211, 199, and 199 genes, respectively, showed ≥ 2.0-fold higher expression than those in the sebaceous adenoma sample (case 4); 194 genes were common to all three SGC samples. For the 194 genes with upregulated expression, functional category analysis showed that SGC of the eyelid employed a unique gene network, including cyclin-dependent kinase inhibitor 2A (CDKN2A), cyclin-dependent kinase 1 (CDK1), and cyclin E1 (CCNE1), which are related to cell cycle progression, incidence of tumor, and cell viability. Furthermore, qRT-PCR analysis showed that the expression levels of CDKN2A, CDK1, and CCNE1 were significantly upregulated in all SGC cases compared to those in the sebaceous adenoma case. These data were similar to the results of microarray analysis. Conclusion: Overexpression of cell cycle-related genes CDKN2A, CDK1, CCNE1, and their gene network may help elucidate the pathogenic pathway of SGC of the eyelid at the molecular level.
AB - Purpose: To investigate the overexpression of genes in sebaceous gland carcinoma (SGC) of the eyelid compared to sebaceous adenoma of the eyelid in order to elucidate the molecular mechanism underlying pathogenesis. Methods: We performed histopathological examination of eyelid tissues surgically removed from four patients diagnosed with SGC (cases 1–3) and sebaceous adenoma (case 4) of the eyelid. Next, we performed global gene expression analysis of surgical tissue samples using a GeneChip® system and the Ingenuity Pathways Knowledge Base. The results of the GeneChip® analysis were explored with quantitative real-time polymerase chain reaction (qRT-PCR) analysis. Results: In the SGC samples, we found that 211, 199, and 199 genes, respectively, showed ≥ 2.0-fold higher expression than those in the sebaceous adenoma sample (case 4); 194 genes were common to all three SGC samples. For the 194 genes with upregulated expression, functional category analysis showed that SGC of the eyelid employed a unique gene network, including cyclin-dependent kinase inhibitor 2A (CDKN2A), cyclin-dependent kinase 1 (CDK1), and cyclin E1 (CCNE1), which are related to cell cycle progression, incidence of tumor, and cell viability. Furthermore, qRT-PCR analysis showed that the expression levels of CDKN2A, CDK1, and CCNE1 were significantly upregulated in all SGC cases compared to those in the sebaceous adenoma case. These data were similar to the results of microarray analysis. Conclusion: Overexpression of cell cycle-related genes CDKN2A, CDK1, CCNE1, and their gene network may help elucidate the pathogenic pathway of SGC of the eyelid at the molecular level.
KW - Cyclin E1
KW - Cyclin-dependent kinase 1
KW - Cyclin-dependent kinase inhibitor 2A
KW - Microarray analysis
KW - Sebaceous gland carcinoma
UR - http://www.scopus.com/inward/record.url?scp=85073987530&partnerID=8YFLogxK
U2 - 10.1007/s10792-019-01185-7
DO - 10.1007/s10792-019-01185-7
M3 - 学術論文
C2 - 31571090
AN - SCOPUS:85073987530
SN - 0165-5701
VL - 40
SP - 343
EP - 350
JO - International Ophthalmology
JF - International Ophthalmology
IS - 2
ER -