Carbon powder-filled microelectrode: An easy-to-fabricate probe for cellular electrochemistry

Asuka Tsujimura; Yuhi Kamae; Hikaru Kawasaki; Haruki Nagai; Masanobu Kano; Toshihide Tabata

doi:10.1016/j.ab.2021.114316

Carbon powder-filled microelectrode: An easy-to-fabricate probe for cellular electrochemistry

Asuka Tsujimura, Yuhi Kamae, Hikaru Kawasaki, Haruki Nagai, Masanobu Kano, Toshihide Tabata^*

^*Corresponding author for this work

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2 Scopus citations

Abstract

Carbon fiber and carbon fiber disc microelectrodes are widely used for electrochemical detection of biochemicals released from cells. However, fabricating these types of microelectrodes is difficult and time-consuming. Here, we report an easy-to-fabricate, carbon powder-filled microelectrode consisting of a pulled glass capillary backfilled with carbon powder. Carbon tip size and responsiveness can be controlled by adjusting the settings of the puller. Carbon powder-filled microelectrodes with tip opening diameters of 7–24 μm detected sub-micromolar to sub-millimolar levels of dopamine and catecholamines released from PC-12 cells. This simple microelectrode should promote further work on cellular and tissue electrochemistry.

Original language	English
Article number	114316
Journal	Analytical Biochemistry
Volume	629
DOIs	https://doi.org/10.1016/j.ab.2021.114316
State	Published - 2021/09/15

Keywords

Amperometry
Cyclic voltammetry
Neuromodulator
Neuron
Neurotransmitter
Synapse

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.ab.2021.114316

Cite this

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title = "Carbon powder-filled microelectrode: An easy-to-fabricate probe for cellular electrochemistry",

abstract = "Carbon fiber and carbon fiber disc microelectrodes are widely used for electrochemical detection of biochemicals released from cells. However, fabricating these types of microelectrodes is difficult and time-consuming. Here, we report an easy-to-fabricate, carbon powder-filled microelectrode consisting of a pulled glass capillary backfilled with carbon powder. Carbon tip size and responsiveness can be controlled by adjusting the settings of the puller. Carbon powder-filled microelectrodes with tip opening diameters of 7–24 μm detected sub-micromolar to sub-millimolar levels of dopamine and catecholamines released from PC-12 cells. This simple microelectrode should promote further work on cellular and tissue electrochemistry.",

keywords = "Amperometry, Cyclic voltammetry, Neuromodulator, Neuron, Neurotransmitter, Synapse",

author = "Asuka Tsujimura and Yuhi Kamae and Hikaru Kawasaki and Haruki Nagai and Masanobu Kano and Toshihide Tabata",

note = "Publisher Copyright: {\textcopyright} 2021",

year = "2021",

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doi = "10.1016/j.ab.2021.114316",

language = "英語",

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journal = "Analytical Biochemistry",

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T1 - Carbon powder-filled microelectrode

T2 - An easy-to-fabricate probe for cellular electrochemistry

AU - Tsujimura, Asuka

AU - Kamae, Yuhi

AU - Kawasaki, Hikaru

AU - Nagai, Haruki

AU - Kano, Masanobu

AU - Tabata, Toshihide

PY - 2021/9/15

Y1 - 2021/9/15

N2 - Carbon fiber and carbon fiber disc microelectrodes are widely used for electrochemical detection of biochemicals released from cells. However, fabricating these types of microelectrodes is difficult and time-consuming. Here, we report an easy-to-fabricate, carbon powder-filled microelectrode consisting of a pulled glass capillary backfilled with carbon powder. Carbon tip size and responsiveness can be controlled by adjusting the settings of the puller. Carbon powder-filled microelectrodes with tip opening diameters of 7–24 μm detected sub-micromolar to sub-millimolar levels of dopamine and catecholamines released from PC-12 cells. This simple microelectrode should promote further work on cellular and tissue electrochemistry.

AB - Carbon fiber and carbon fiber disc microelectrodes are widely used for electrochemical detection of biochemicals released from cells. However, fabricating these types of microelectrodes is difficult and time-consuming. Here, we report an easy-to-fabricate, carbon powder-filled microelectrode consisting of a pulled glass capillary backfilled with carbon powder. Carbon tip size and responsiveness can be controlled by adjusting the settings of the puller. Carbon powder-filled microelectrodes with tip opening diameters of 7–24 μm detected sub-micromolar to sub-millimolar levels of dopamine and catecholamines released from PC-12 cells. This simple microelectrode should promote further work on cellular and tissue electrochemistry.

KW - Amperometry

KW - Cyclic voltammetry

KW - Neuromodulator

KW - Neuron

KW - Neurotransmitter

KW - Synapse

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DO - 10.1016/j.ab.2021.114316

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JO - Analytical Biochemistry

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Carbon powder-filled microelectrode: An easy-to-fabricate probe for cellular electrochemistry

Abstract

Keywords

ASJC Scopus subject areas

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