Development of a novel, rapid, easy and affordable identification IT system for pathogens in sepsis

Project Details

Description

To detect pathogenic bacteria by PCR sensitively and correctly, we newly developed 'eukaryote-made' Taq polymerase, which is free from bacterial DNA contamination(J Clin Microbiol. 2011 Sep ; 49(9): 3316-20). We also developed a novel rapid method to identify pathogenic microorganisms using 7 primer sets, real-time PCR, high resolution melting analysis(RotorGeneQ : QIAGEN), and the original web-based identification software(International patent application : 2007). To combine the eukaryote-made Taq polymerase and a novel identification method, we developed a rapid identification system of pathogenic microorganisms within 3 hours after collection of patient samples.
StatusFinished
Effective start/end date2009/04/012012/03/31

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